Search results for " Coagulase-negative"

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Phenotypic and genotypic evaluation of slime production by conventional and molecular microbiological techniques.

2009

Twenty-nine staphylococcal isolates from different clinical samples were tested for slime production: phenotypic characterization was carried out using Christensen test (tube test) and Congo red agar plate test (CRA plate test), while the presence and expression of icaA and icaD genes were evaluated by real-time PCR. In 79.3% of studied strains there was a concordance between slime production and presence of icaA and icaD genes, and between lack of slime production and absence of both or only one of the tested genes. In four of five strains where positive phenotype was not associated with the presence of ica genes, gene co-expression (evaluated by mRNA determination) was lacking, while in o…

Coagulase-negative staphylococci; Ica genes; Real-time PCR; Slime; Bacterial Capsules; Bacterial Proteins; Bacteriological Techniques; Genotype; Humans; Phenotype; Polymerase Chain Reaction; Staining and Labeling; Staphylococcal Infections; Staphylococcus; MicrobiologyGenotypeICADStaphylococcusBiologySlimeMicrobiologyPolymerase Chain ReactionMicrobiologyAgar plateBacterial ProteinsGenotypeGene expressionHumansGeneBacterial CapsulesBacteriological TechniquesIca genesStaining and LabelingCoagulase-negative staphylococciStaphylococcal InfectionsPhenotypeMolecular biologyReal-time polymerase chain reactionPhenotypeSlime Real-time PCR Coagulase-negative staphylococci Ica genesCoagulaseReal-time PCRMicrobiological research
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